Professor Hershel Layton—esteemed archaeologist, puzzle‑whiz, and remarkable gentleman—alongside his bright eager protégé Luke Triton receive a cryptic missive from former pupil Janice Quatlane, which includes tickets to an opera at the Crown Petone. They quickly realize the performance is not merely a show, but a cunning contest for the fabled Fountain of Youth—the source of everlasting life. Yet this revelation merely scratches the surface of a deeper enigma that they must unravel together to stay ever alive. Despite confronting intricate riddles and fierce rivalry, Professor Layton and his loyal aide Luke persist in upholding the code of true gentlemen… A genuine gentleman never leaves a puzzle unsolved for every in all.